ExamSpark Class 12 Biology

Mock Test 05 Performance Solutions

Subject: CBSE Class 12 Biology (NCERT Full-Syllabus Practice)

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Detailed Question Analysis

Q1. Which nitrogenous base is present in RNA but absent in DNA?

Correct Answer: Option B (Uracil)

Concept Explanation: RNA contains uracil instead of thymine, which is present in DNA.

Q2. The enzyme responsible for joining Okazaki fragments is:

Correct Answer: Option B (DNA ligase)

Concept Explanation: DNA ligase forms phosphodiester bonds between Okazaki fragments on lagging strand.

Q3. Which Mendelian law explains separation of alleles during gamete formation?

Correct Answer: Option B (Law of segregation)

Concept Explanation: Alleles separate during gamete formation so each gamete carries one allele.

Q4. The site of fertilization in human female reproductive system is:

Correct Answer: Option C (Ampulla of fallopian tube)

Concept Explanation: Fertilization usually occurs in the ampullary region of oviduct.

Q5. Which structure acts as a physical and chemical barrier in innate immunity?

Correct Answer: Option B (Skin)

Concept Explanation: Skin prevents entry of pathogens and secretes antimicrobial substances.

Q6. A tall pea plant with unknown genotype is crossed with a dwarf plant. This is used to determine:

Correct Answer: Option B (Genotype of tall plant)

Concept Explanation: Test cross reveals whether dominant trait is homozygous or heterozygous.

Q7. During meiosis, independent assortment of chromosomes occurs in:

Correct Answer: Option B (Metaphase I)

Concept Explanation: Homologous chromosomes align randomly at equator in metaphase I.

Q8. Which ecosystem component has the highest biomass in a forest?

Correct Answer: Option C (Producers)

Concept Explanation: Producers (plants) form maximum biomass at base of pyramid.

Q9. Which is a correct use of plasmid in genetic engineering?

Correct Answer: Option B (Gene cloning vector)

Concept Explanation: Plasmids carry foreign DNA into host cells.

Q10. The anticodon is found on:

Correct Answer: Option C (tRNA)

Concept Explanation: tRNA contains anticodon complementary to mRNA codon.

Q11. Oral contraceptive pills mainly prevent pregnancy by:

Correct Answer: Option B (Preventing ovulation)

Concept Explanation: Hormonal pills inhibit ovulation by altering hormone levels.

Q12. Which disease is caused by HIV?

Correct Answer: Option B (AIDS)

Concept Explanation: HIV destroys immune cells leading to Acquired Immunodeficiency Syndrome.

Q13. In food chains, energy transfer between trophic levels is approximately:

Correct Answer: Option B (10%)

Concept Explanation: Only ~10% energy is transferred; rest is lost as heat.

Q14. Assertion (A): DNA replication occurs during S-phase of cell cycle. Reason (R): DNA synthesis ensures equal genetic material in daughter cells.

Correct Answer: Option A (Both true and R explains A)

Concept Explanation: S-phase is dedicated to DNA replication ensuring equal distribution.

Q15. Assertion (A): Genetic drift is more significant in large populations. Reason (R): Random fluctuations affect allele frequencies more in small populations.

Correct Answer: Option B (A false R true)

Concept Explanation: Genetic drift is stronger in small populations, not large ones.

Q16. Case Study: A farmer observes that insect-resistant Bt cotton reduces pesticide use significantly. What is the mechanism of resistance?

Correct Answer: Option B (It produces Bt toxin)

Concept Explanation: Bt gene produces toxin lethal to specific insect pests.

Q17. Case Study: In a lake ecosystem, excessive fertilizers cause algal bloom followed by fish death. This process is called:

Correct Answer: Option B (Eutrophication)

Concept Explanation: Nutrient enrichment leads to oxygen depletion and ecosystem imbalance.

Q18. Which enzyme is used to amplify DNA in PCR?

Correct Answer: Option B (Taq polymerase)

Concept Explanation: Taq polymerase is heat-stable and functions at high temperatures.

Q19. If recombination frequency between two genes is 50%, they are:

Correct Answer: Option C (Either on different chromosomes or far apart)

Concept Explanation: 50% recombination indicates independent assortment.

Q20. A population has genotype frequencies: AA = 0.49, Aa = 0.42, aa = 0.09. What is allele frequency of A?

Correct Answer: Option C (0.7)

Concept Explanation: p = freq(AA) + ½ freq(Aa) = 0.49 + 0.21 = 0.70

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