ExamSpark Class 12 Biology

Mock Test 03 Performance Solutions

Subject: CBSE Class 12 Biology (NCERT Full-Syllabus Practice)

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Detailed Question Analysis

Q1. During transcription in eukaryotes, which enzyme is directly responsible for synthesis of mRNA?

Correct Answer: Option B (RNA polymerase II)

Concept Explanation: RNA polymerase II synthesizes hnRNA (precursor of mRNA) in eukaryotes. Other polymerases synthesize rRNA or have different roles.

Q2. During translation, the correct order of events is:

Correct Answer: Option B (Initiation \(\rightarrow\) Elongation \(\rightarrow\) Termination)

Concept Explanation: Protein synthesis begins with initiation, continues with elongation of polypeptide chain, and ends with termination at stop codon.

Q3. Which immunoglobulin is most abundant in human blood serum?

Correct Answer: Option C (IgG)

Concept Explanation: IgG constitutes about 75% of serum antibodies and provides long-term immunity.

Q4. If allele frequency in a population remains constant generation after generation, this condition is known as:

Correct Answer: Option C (Hardy-Weinberg equilibrium)

Concept Explanation: Hardy-Weinberg equilibrium states that allele frequencies remain constant in absence of evolutionary forces.

Q5. Double fertilization in angiosperms results in formation of:

Correct Answer: Option B (Embryo and endosperm)

Concept Explanation: One male gamete fuses with egg (zygote), another with polar nuclei (endosperm).

Q6. In a pedigree, a trait appears only in males and is passed from mother to sons. The most likely inheritance is:

Correct Answer: Option B (X-linked recessive)

Concept Explanation: X-linked recessive traits often appear in males and are transmitted through carrier mothers.

Q7. A cloning vector must have which essential feature?

Correct Answer: Option B (Origin of replication)

Concept Explanation: Ori (origin of replication) is essential for replication of vector inside host cell.

Q8. In an ecosystem, which pyramid is always upright?

Correct Answer: Option C (Pyramid of energy)

Concept Explanation: Energy decreases at each trophic level due to loss as heat, so energy pyramid is always upright.

Q9. Restriction enzymes recognize:

Correct Answer: Option C (Specific palindromic DNA sequences)

Concept Explanation: Restriction endonucleases cut DNA at specific palindromic recognition sites.

Q10. In PCR, which component is NOT directly required?

Correct Answer: Option D (Ribosomes)

Concept Explanation: Ribosomes are not involved in PCR; it is an in vitro DNA amplification process.

Q11. Vaccination helps in disease prevention by:

Correct Answer: Option C (Stimulating memory immune response)

Concept Explanation: Vaccines prepare immune system to respond faster upon real infection.

Q12. In diabetes mellitus, which hormone is deficient or ineffective?

Correct Answer: Option B (Insulin)

Concept Explanation: Insulin regulates blood glucose; its deficiency leads to hyperglycemia.

Q13. Excess nutrients in water bodies lead to:

Correct Answer: Option B (Eutrophication)

Concept Explanation: Nutrient overload causes algal bloom and oxygen depletion.

Q14. Assertion (A): Crossing over occurs during pachytene stage of prophase I. Reason (R): It increases genetic variation in offspring.

Correct Answer: Option A (Both A and R are true and R explains A)

Concept Explanation: Crossing over occurs in pachytene and leads to recombination, increasing variation.

Q15. Assertion (A): Lac operon is induced in absence of lactose. Reason (R): Repressor binds to operator and blocks transcription.

Correct Answer: Option D (A false R true)

Concept Explanation: Lac operon is OFF without lactose because repressor blocks transcription.

Q16. Case Study: A patient shows uncontrolled cell division forming a mass of cells that can spread to other organs. What is the most likely condition?

Correct Answer: Option B (Cancer)

Concept Explanation: Uncontrolled proliferation and metastasis are characteristic of cancer.

Q17. Case Study: In recombinant insulin production, human insulin gene is inserted into bacteria which then produce insulin. Why is E. coli commonly used?

Correct Answer: Option C (It has well-known genetics and rapid multiplication)

Concept Explanation: E. coli is easy to manipulate and multiplies rapidly, making it ideal for recombinant protein production.

Q18. If recombination frequency between two genes is 20%, they are:

Correct Answer: Option C (Closely linked)

Concept Explanation: Lower recombination frequency indicates genes are closely located on same chromosome.

Q19. A bacterial population becomes resistant to antibiotics over time due to:

Correct Answer: Option B (Natural selection)

Concept Explanation: Antibiotic-resistant bacteria survive and reproduce, leading to natural selection.

Q20. A population has 16% individuals showing recessive phenotype (aa). What is frequency of dominant allele (A)?

Correct Answer: Option B (0.6)

Concept Explanation: q² = 0.16 \(\rightarrow\) q = 0.4 \(\rightarrow\) p = 1 - 0.4 = 0.6

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